1. Overview

TRO Reagents are installed inside the TRO Sensor Box within the TSU (TRO Sensor Unit).

📌 Reagent Shelf Life:

• Buffer: Up to 1 year after opening.
• Indicator: Up to 3 months after opening (if cooled by a cooling chamber).

📌 Storage Recommendations:

• Before opening: Store in a shaded, cool place (around 24°C).
• After opening (if unused): Store in a refrigerator, but DO NOT store below 4°C.

📌 Replacement Conditions:

• Expired reagent or color change requires replacement.
  • Normal State: Transparent or light pink.
  • Abnormal State: Dark pink, navy, or black.
    

📌 Purchasing Reagents:

• TRO Reagents are consumables.
• For purchase inquiries, contact hanla@hanlaims.com.

2. Pre-Replacement Preparation

1. Press the "SERVICE" button once to stop seawater flow and empty the Cuvette.

3. Buffer Replacement Procedure

1. Replace the existing Buffer with a newly prepared one.
   
2. Ensure the reagent tube under the cap is correctly positioned and fully submerged in the reagent to prevent suction failure.
3. Record the replacement date.

4. Indicator Replacement Procedure

1. Shake the DPD powder thoroughly to ensure it is completely powdery.
   
2. Add the DPD powder to the Indicator solution.
3. Shake the Indicator bottle until the powder is fully dissolved.
   
4. Replace the existing Indicator with the newly prepared one.
5. Ensure the reagent tube under the cap is correctly positioned and fully submerged in the reagent to prevent suction failure.
6. Record the replacement date.

5. Priming the Reagents

1. After reagent replacement, press the "PRIME" button.
2. Press the "↵ (Enter)" button.
   
3. The reagent pump will activate, injecting reagent into the Cuvette.
   
4. Repeat priming until all air disappears from the reagent tube.

6. Flushing the Reagent Tube (If Tube is Clogged or Air Remains)

1. If the tube appears clogged or air remains in the reagent tube, perform flushing:
   1. Clean the injector with clean water.
   2. Aspirate Buffer or Indicator into a syringe.
      
   3. Inject the aspirated reagent into the reagent tube under the cap of the Buffer or Indicator.
      
   4. Verify that the injected reagent flows into the cuvette.
   5. After flushing, restore the components to their original state.

7. Additional Notes

1. If the TRO Sensor is installed in the Pump Room or Upper Deck, it may be an explosion-proof model.
   • Before opening the TRO Sensor door, turn the Air Sentinel key clockwise to switch to BYPASS mode.
     
2. After closing the TRO Sensor door, return the Air Sentinel key to its original state.

8. Summary of Key Actions